Journal: Horticulture Research

Article Title: Spatiotemporal miRNA and transcriptomic network dynamically regulate the developmental and senescence processes of poplar leaves

doi: 10.1093/hr/uhad186

Figure Lengend Snippet: Sample collection and cluster analysis of the poplar leaf transcriptome and miRNAs a Fifteen leaf positions were collected for RNA-seq, and seven leaf positions were collected for small RNA sequencing. b 3D PCA of the transcriptomes of all 15 sampling points. c – e Phenotype (photosynthesis rate, leaf area, content of chlorophyll) of poplar leaf at the Y–M stage and M–S stage.

Article Snippet: The chlorophyll content index and leaf area were measured using a CCM-200 Plus chlorophyll content meter (Opti-Sciences Inc., USA) and a CI-203 portable laser leaf area meter.

Techniques: RNA Sequencing, Sampling

Journal: Horticulture Research

Article Title: Spatiotemporal miRNA and transcriptomic network dynamically regulate the developmental and senescence processes of poplar leaves

doi: 10.1093/hr/uhad186

Figure Lengend Snippet: Role of core transcription factors in the regulation of leaf development. a Heat map of GRF9 and MYB5 in leaf development. b Subcellular localization analysis of MYB5. c , d Coding sequence and protein sequence analysis of MYB5. e Identification of overexpression of MYB5 by PCR. f Phenotype of OEMYB5. g – i Quantitative determination of content of chlorophyll a, chlorophyll b, and proanthocyanidin in PpnMyb5-OE plants. j Quantitative reverse transcription–PCR analysis of MYB5 and PAL1, DFR, FLS1, F3H, TT8, LDOX, and TT4 genes in the wild type.

Article Snippet: The chlorophyll content index and leaf area were measured using a CCM-200 Plus chlorophyll content meter (Opti-Sciences Inc., USA) and a CI-203 portable laser leaf area meter.

Techniques: Sequencing, Over Expression, Reverse Transcription